5DTX
Crystal structure of rsEGFP2 in the fluorescent on-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.990, 62.910, 70.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.039 - 1.450 |
| R-factor | 0.1759 |
| Rwork | 0.174 |
| R-free | 0.20610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.653 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.040 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 40827 | |
| <I/σ(I)> | 16.1 | 2.3 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 0.1 M HEPES, 1.7 M ammonium sulphate |
