5DTL
Crystal structure of mEos2-A69T fluorescent protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.370, 96.500, 100.060 |
| Unit cell angles | 91.68, 107.83, 97.38 |
Refinement procedure
| Resolution | 95.450 - 2.700 |
| R-factor | 0.21189 |
| Rwork | 0.211 |
| R-free | 0.23151 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s05 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.195 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.450 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 67723 | |
| <I/σ(I)> | 6.9 | 1.8 |
| Completeness [%] | 95.4 | 95.48 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.2 M chloride hexahydrate, 0.1 M Tris, 30% w/v polyethylene glycol 3,350 |
