5DTB
Crystal structure of the Drosophila CG3822 KaiR1D ligand binding domain complex with glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 87.704, 87.704, 183.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.801 - 1.843 |
| R-factor | 0.1632 |
| Rwork | 0.162 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s50 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2124)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.850 |
| Rmerge | 0.070 | 0.595 |
| Number of reflections | 62542 | |
| <I/σ(I)> | 26.3 | 2.05 |
| Completeness [%] | 97.9 | 96.9 |
| Redundancy | 8.2 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Buffer 150 NaCl 10 Tris, 2 Glu, 1 EDTA Reservoir 10% PEG 8K 0.1 Bicine pH 9, 2% Dioxane |
