5DSZ

Gamma-subunit of the translation initiation factor 2 from S. solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	203.3
Detector technology	CCD
Collection date	2007-06-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.8148
Spacegroup name	P 31
Unit cell lengths	95.430, 95.430, 164.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.858 - 2.500
R-factor	0.2018
Rwork	0.201
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2plf
RMSD bond length	0.012
RMSD bond angle	1.567
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_1389)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.858	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.081	0.583
Number of reflections	52390
<I/σ(I)>	7.56
Completeness [%]	90.4	98.6
Redundancy	2.85	2.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	15% NATRIUM MALONATE DIHYDRATE, 50MM GLYCINE, 13MM CDCL2 AT THE PROTEIN/NUCLEOTIDE MOLAR RATIO 1/10. FOR CRYOPROTECTION ETHYLENE GLYCOL WAS ADDED TO THE RESERVOIR SOLUTION AT THE FINAL CONCENTRATION OF 15% (V/V)