5DSU
Crystal structure of double mutant of N-domain of human calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 38.400, 38.400, 81.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.992 - 1.930 |
| R-factor | 0.2303 |
| Rwork | 0.229 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cll |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.312 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.992 | 2.170 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 5544 | |
| <I/σ(I)> | 6.8 | 2.1 |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 4.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 37% PEG 400, 100 mM HEPES, 250 MM CaCl2 |
