5DSS
MP-4 contributes to snake venom neutralization by Mucuna pruriens seeds through stimulation of cross-reactive antibodies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.000, 69.180, 45.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.910 - 2.800 |
| R-factor | 0.26762 |
| Rwork | 0.267 |
| R-free | 0.28439 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r8n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.645 |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.910 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.073 | |
| Number of reflections | 4048 | |
| <I/σ(I)> | 25.6 | 14.5 |
| Completeness [%] | 95.5 | 94 |
| Redundancy | 9.7 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 50 mM Tris-HCl pH 9.5, 1.5 M ammonium sulfate |
