5DSB

Crystal structure of Holliday junctions stabilized by 5-hydroxymethylcytosine in GCC junction core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-28
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	66.073, 24.768, 38.061
Unit cell angles	90.00, 111.82, 90.00

Refinement procedure

Resolution	35.335 - 1.496
R-factor	0.2528
Rwork	0.251
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p4y
RMSD bond length	0.011
RMSD bond angle	1.276
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.496	4.070	1.496
Rmerge	0.117	0.109	0.639
Rmeas	0.123	0.113	0.802
Rpim	0.036	0.031	0.478
Total number of observations	62380
Number of reflections	9346
<I/σ(I)>	14.9
Completeness [%]	99.3	100	94.3
Redundancy	6.7	13.9	2.2
CC(1/2)		0.988	0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.78 mM DNA, 0.8 mM Spermine, 5 mM CaCl2, 25 mM sodium cacodylate