5DS1
Core domain of the class II small heat-shock protein HSP 17.7 from Pisum sativum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-03 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.860, 82.690, 88.900 |
| Unit cell angles | 90.00, 92.11, 90.00 |
Refinement procedure
| Resolution | 14.979 - 2.630 |
| R-factor | 0.2374 |
| Rwork | 0.231 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ds2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.134 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.020 | 2.724 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 10426 | |
| <I/σ(I)> | 7.34 | 1.95 |
| Completeness [%] | 98.5 | 93.7 |
| Redundancy | 3.53 | 2.81 |
| CC(1/2) | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | c17.7 was crystallized in 200 mM CaCl2, 100 mM sodium cacodylate pH 6.5, 40 % (v/v) polyethylene glycol (PEG) 200 in hanging drop plates at room temperature. |
