5DRW
Crystal structure of the BCR Fab fragment from subset #4 case CLL183
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 90.210, 90.210, 131.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.660 - 2.270 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dn0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.660 | 2.340 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.113 | 1.205 |
| Number of reflections | 25817 | |
| <I/σ(I)> | 10.7 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.6 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 0.1 M NaCitrate, 0.1 M ammonium sulfate, 1% hexanediol, 14% PEG 3350 |
