5DRA
t3284 loop variant of beta1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 48.340, 48.340, 221.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.227 - 3.000 |
| R-factor | 0.2522 |
| Rwork | 0.250 |
| R-free | 0.30710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.446 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.035 | 0.098 |
| Number of reflections | 5854 | |
| <I/σ(I)> | 33.07 | 14.06 |
| Completeness [%] | 98.8 | 98.5 |
| Redundancy | 12.1 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 290 | 1.1M sodium acetate pH 4.6 |
