5DQZ
Crystal Structure of Cas-DNA-PAM complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.771, 193.380, 193.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.534 - 2.700 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p6i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.979 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.113 | 0.044 | |
| Total number of observations | 590102 | ||
| Number of reflections | 76448 | ||
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 99.5 | 96.2 | 99.6 |
| Redundancy | 7.7 | 7.1 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 50mM Na cacodylate,0.9mM spermine,18mM Magnesium chloride, 1.8mM Cobalt chloride, 9% isopropanol |
