5DQW
The crystal structure of Bacillus subtilis YpgQ in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.865, 52.499, 82.322 |
| Unit cell angles | 90.00, 93.33, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.21209 |
| Rwork | 0.209 |
| R-free | 0.26134 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dqv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.441 | |
| Number of reflections | 22139 | |
| <I/σ(I)> | 34 | 3.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 40% PEG 600, 0.1 M sodium citrate |
