5DQV
The crystal structure of Bacillus subtilis YpgQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97956 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.061, 53.432, 82.610 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.19026 |
| Rwork | 0.188 |
| R-free | 0.22753 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.507 | |
| Number of reflections | 27729 | |
| <I/σ(I)> | 30.4 | 3.2 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 38% PEG 600, 0.1 M MES |
