5DQS

Complex structure of human elongation factor 1B alpha and gamma GST-like domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	293.15
Detector technology	CCD
Collection date	2015-07-25
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	50.788, 50.788, 187.439
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.984 - 2.100
R-factor	0.2079
Rwork	0.204
R-free	0.24160
RMSD bond length	0.004
RMSD bond angle	0.802
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.984
High resolution limit [Å]	2.100
Number of reflections	31057
<I/σ(I)>	54.1
Completeness [%]	98.6
Redundancy	17.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	glycerol ethoxylate