5DQK
Two divalent metal ions and conformational changes play roles in the hammerhead ribozyme cleavage reaction-WT ribozyme in Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 79.764, 86.070, 103.338 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.069 - 2.707 |
R-factor | 0.2296 |
Rwork | 0.224 |
R-free | 0.27780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zd5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.378 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.059 | 0.785 |
Number of reflections | 9951 | |
<I/σ(I)> | 28 | 2.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 8.1 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 33% MPD, 0.4 M potassium chloride, 50 mM potassium acetate pH 5.0, 10 mM MgCl2 and 0.5mM spermine |