5DQE

Crystal structure of human transcription factor TEAD2 in complex with bromo-fenamic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.91925
Spacegroup name	C 1 2 1
Unit cell lengths	121.470, 61.582, 80.419
Unit cell angles	90.00, 117.70, 90.00

Refinement procedure

Resolution	38.669 - 2.183
R-factor	0.1812
Rwork	0.179
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l15
RMSD bond length	0.015
RMSD bond angle	1.498
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: dev_1951))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.220
High resolution limit [Å]	2.180	2.180
Number of reflections	24263
<I/σ(I)>	24	3.14
Completeness [%]	98.4	86.6
Redundancy	4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	0.1 M HEPES pH 7.2 and 2.4 M sodium formate.