5DQ6

Mus musculus A20 OTU domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 32
Unit cell lengths	71.340, 71.340, 143.181
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.770 - 2.800
R-factor	0.2555
Rwork	0.253
R-free	0.29050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zjf
RMSD bond length	0.011
RMSD bond angle	1.434
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.770
High resolution limit [Å]	2.800
Rmerge	0.066
Number of reflections	19279
<I/σ(I)>	11.4
Completeness [%]	92.1
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2.4 M NaCl, 0.1 M MES