5DPW
Crystal structure of PLEKHM1 LIR in complex with human LC3C_8-125
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.140, 73.225, 89.048 |
| Unit cell angles | 89.96, 89.99, 77.31 |
Refinement procedure
| Resolution | 38.610 - 2.185 |
| R-factor | 0.2006 |
| Rwork | 0.200 |
| R-free | 0.24570 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.798 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.610 | 2.240 |
| High resolution limit [Å] | 2.185 | 2.190 |
| Number of reflections | 68176 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 98.6 | 97.8 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | 0.2 M Potassium Phosphate monobasic, 20% w/v Polyethylene glycol 3,350 |
