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5DPJ

sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-E
Synchrotron siteAPS
Beamline24-ID-E
Temperature [K]100
Detector technologyCCD
Collection date2013-11-15
DetectorADSC QUANTUM 315
Wavelength(s)0.98
Spacegroup nameP 1 21 1
Unit cell lengths46.288, 112.935, 93.413
Unit cell angles90.00, 104.09, 90.00
Refinement procedure
Resolution47.920 - 2.500
R-factor0.184
Rwork0.180
R-free0.24400
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2b3p
RMSD bond length0.010
RMSD bond angle1.524
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.540
High resolution limit [Å]2.4952.500
Rmerge0.0840.548
Number of reflections32214
<I/σ(I)>10.91.4
Completeness [%]99.398.9
Redundancy1.91.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP729822% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7

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PDB entries from 2024-05-15

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