5DPJ
sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.288, 112.935, 93.413 |
Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
Resolution | 47.920 - 2.500 |
R-factor | 0.184 |
Rwork | 0.180 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.524 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.495 | 2.500 |
Rmerge | 0.084 | 0.548 |
Number of reflections | 32214 | |
<I/σ(I)> | 10.9 | 1.4 |
Completeness [%] | 99.3 | 98.9 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7 |