5DPJ
sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.288, 112.935, 93.413 |
| Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
| Resolution | 47.920 - 2.500 |
| R-factor | 0.184 |
| Rwork | 0.180 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.524 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.495 | 2.500 |
| Rmerge | 0.084 | 0.548 |
| Number of reflections | 32214 | |
| <I/σ(I)> | 10.9 | 1.4 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7 |
