5DPI
sfGFP double mutant - 133/149 p-cyano-L-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.945, 59.067, 131.761 |
| Unit cell angles | 90.00, 108.78, 90.00 |
Refinement procedure
| Resolution | 43.865 - 2.540 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.24350 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.109 | 0.700 |
| Number of reflections | 58961 | |
| <I/σ(I)> | 14.7 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M potassium sodium formate, 30% PEG 3350 |
