5DP1
Crystal structure of CurK enoyl reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 93.583, 127.153, 127.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.300 - 1.850 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.22004 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vz9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.300 |
| High resolution limit [Å] | 1.850 |
| Rmerge | 0.059 |
| Number of reflections | 32609 |
| <I/σ(I)> | 21 |
| Completeness [%] | 99.0 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.2 M Li2SO4 and 0.1 M CAPS pH 10.5 |
