5DOR
P2 Integrase catalytic domain in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2782 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.110, 92.225, 64.937 |
| Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
| Resolution | 64.540 - 2.500 |
| R-factor | 0.2318 |
| Rwork | 0.230 |
| R-free | 0.27520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c6k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.462 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.540 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.077 | 0.759 |
| Number of reflections | 26038 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Zinc Chloride, Tris, PEG 6000 |
