5DOQ
The structure of bd oxidase from Geobacillus thermodenitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.738290 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 110.060, 120.860, 122.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.050 |
| R-factor | 0.32015 |
| Rwork | 0.319 |
| R-free | 0.33472 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.120 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.099 | 2.200 |
| Number of reflections | 18373 | |
| <I/σ(I)> | 19.17 | 3.03 |
| Completeness [%] | 56.7 | 2.6 |
| Redundancy | 31.44 | 19.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 400, Ammonium sulfate, Na-HEPES |
