5DOM
Crystal structure, maturation and flocculating properties of a 2S albumin from Moringa oleifera seeds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.45 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 106.867, 106.867, 43.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.570 - 1.690 |
| R-factor | 0.22391 |
| Rwork | 0.222 |
| R-free | 0.26288 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.018 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.810 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 13674 | |
| <I/σ(I)> | 16.96 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.3 | 293 | Initial: 0.1 M sodium acetate pH 4.6 and 2.0 M NaCl, Optimization: 0.1 M sodium acetate pH 4.3 and 2.4 M NaCl |
