5DOE
Crystal structure of the Human Cytomegalovirus UL53 (residues 72-292)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0703 |
| Spacegroup name | P 4 |
| Unit cell lengths | 90.008, 90.008, 33.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 3.000 |
| R-factor | 0.25551 |
| Rwork | 0.253 |
| R-free | 0.30879 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.101 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 90.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 5574 |
| <I/σ(I)> | 14.2 |
| Completeness [%] | 97.8 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280.65 | 0.1 M Tris, 0.1 M NaCl, 20-30% polyethylene glycol (PEG) 3350 (w/v) and 10 % glycerol |
