5DNP
Crystal structure of Mmi1 YTH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.490, 58.353, 54.266 |
| Unit cell angles | 90.00, 108.19, 90.00 |
Refinement procedure
| Resolution | 49.790 - 2.300 |
| R-factor | 0.2151 |
| Rwork | 0.212 |
| R-free | 0.25550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3i |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.187 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.790 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.128 | 0.685 |
| Number of reflections | 13290 | |
| <I/σ(I)> | 13 | 2.89 |
| Completeness [%] | 99.3 | 96.8 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES (pH 6.0), 18% (w/v) PEG 2000 |
