5DNJ

Mouse Polo-box domain and Peptide analog 702

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-20
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	57.149, 59.989, 67.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.700 - 2.300
R-factor	0.19983
Rwork	0.196
R-free	0.27693
RMSD bond length	0.014
RMSD bond angle	1.820
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.700
High resolution limit [Å]	2.300
Number of reflections	9156
<I/σ(I)>	23.4
Completeness [%]	90.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	278	28% 2-propanol, 3% PEG 200, 0.1 M Bis-Tris (pH 6.5)