5DNB
STRUCTURE OF THE B-DNA DECAMER C-C-A-A-C-G-T-T-G-G AND COMPARISON WITH ISOMORPHOUS DECAMERS C-C-A-A-G-A-T-T-G-G AND C-C-A-G-G-C-C-T-G-G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 273 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 32.250, 25.530, 34.380 |
| Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.400 |
| R-factor | 0.16 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.050 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 4402 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 10 (mg/ml) | |
| 2 | 1 | drop | 0.76 (M) | ||
| 3 | 1 | reservoir | MPD | 60 (%) |
