5DN4
Structure of the glycoside hydrolase domain from Salmonella typhimurium FlgJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.790, 43.630, 107.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.989 - 1.800 |
| R-factor | 0.1835 |
| Rwork | 0.182 |
| R-free | 0.21150 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.500 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.690 |
| Number of reflections | 17650 | |
| <I/σ(I)> | 12.9 | 3 |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 13.3 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 35 mg/ml protein in 18-22% polyethylene glycol 3350 and 0.25-0.35 M NaI |
