5DN2
Human NRP2 b1 domain in complex with the peptide corresponding to the C-terminus of VEGF-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.250, 139.520, 106.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.500 - 1.950 |
| R-factor | 0.20576 |
| Rwork | 0.204 |
| R-free | 0.23368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qqj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.463 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.500 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.059 | 0.223 |
| Number of reflections | 59661 | |
| <I/σ(I)> | 10.8 | 4.3 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289 | 10% PEG20000, 0.1M Bicine, 2% v/v dioxane |
