5DN0
t1555 loop variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 108.270, 108.270, 151.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.010 - 4.400 |
| R-factor | 0.25728 |
| Rwork | 0.254 |
| R-free | 0.33089 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.564 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.010 | 4.510 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Rmerge | 0.088 | 0.551 |
| Number of reflections | 3058 | |
| <I/σ(I)> | 14.3 | 3.6 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 8.2 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 290 | 30% v/v glycerol pH7, 1.26M ammonium dihydrogen phosphate |
