5DMQ
Crystal structure of mouse eRF1 in complex with Reverse Transcriptase (RT) of Moloney Murine Leukemia Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.653, 92.653, 289.949 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.650 - 4.000 |
| R-factor | 0.24367 |
| Rwork | 0.241 |
| R-free | 0.29523 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.316 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.650 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.100 | 0.700 |
| Number of reflections | 12901 | |
| <I/σ(I)> | 6.9 | 2.5 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.02 M magnesium chloride, 0.1 M HEPES pH 7.5, 20 % polyacrylic acid sodium salt 5100 |
