5DMH

Crystal structure of a domain of unknown function (DUF1537) from Ralstonia eutropha H16 (H16_A1561), Target EFI-511666, complex with ADP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-12
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	53.130, 87.666, 178.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.365 - 1.800
R-factor	0.1546
Rwork	0.153
R-free	0.18530
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.282
Data scaling software	Aimless (0.1.27)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.365	178.990	1.840
High resolution limit [Å]	1.800	9.180	1.800
Rmerge	0.103	0.056	0.857
Rpim	0.029	0.018	0.271
Total number of observations	996969	7543	48046
Number of reflections	78487
<I/σ(I)>	17.3	39	2.7
Completeness [%]	100.0	97.5	100
Redundancy	12.7	10.9	10.8
CC(1/2)	0.999	0.997	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	Protein (30 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 10 mM ATP, 5 mM MgCl2); Reservoir (MCSG1 (H1) - 0.2 M Ammonium Fluoride, 20%(w/v) PEG 3350); Cryoprotection (80% Reservoir, 20% glycerol)