5DMB
Crystal structure of a translational regulator bound to a flagellar assembly factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.966 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.876, 61.681, 42.902 |
| Unit cell angles | 90.00, 98.08, 90.00 |
Refinement procedure
| Resolution | 42.477 - 2.301 |
| R-factor | 0.2275 |
| Rwork | 0.224 |
| R-free | 0.28810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aj7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.396 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1685) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.480 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.056 | 0.518 |
| Number of reflections | 11552 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M potassium fluoride, 20 % (w/v) PEG 3350 |
