5DKU
C-terminal His tagged apPOL exonuclease mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 65 |
| Unit cell lengths | 146.382, 146.382, 165.671 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.915 - 2.900 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dkt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.477 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX ((1.10_2148: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.094 | 0.032 | 0.526 |
| Rmeas | 0.110 | 0.037 | 0.625 |
| Rpim | 0.056 | 0.018 | 0.328 |
| Total number of observations | 155640 | ||
| Number of reflections | 44314 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 99.3 | 98.1 | 98.6 |
| Redundancy | 3.5 | 3.7 | 3.2 |
| CC(1/2) | 0.998 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trhydrate pH 6.5, 30% w/v polyethylene glycol 4000 |
