5DJX

Fc Heterodimer Design 2.9 L368M/K370E + E357A/S364G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2013-10-07
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 1
Unit cell lengths	59.880, 65.605, 78.282
Unit cell angles	88.97, 76.34, 88.00

Refinement procedure

Resolution	30.000 - 2.250
R-factor	0.20552
Rwork	0.203
R-free	0.25645
RMSD bond length	0.006
RMSD bond angle	1.111
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	76.064	2.370
High resolution limit [Å]	2.250	7.120	2.250
Rmerge		0.040	0.526
Rmeas	0.142
Rpim	0.085	0.029	0.413
Total number of observations	139537	4273	20398
Number of reflections	53335
<I/σ(I)>	6.9	17	2
Completeness [%]	97.1	96.2	96.6
Redundancy	2.6	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	5% Glycerol + 15% PEG 6K