5DJ5

Crystal structure of rice DWARF14 in complex with synthetic strigolactone GR24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	48.400, 87.880, 118.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.920 - 2.400
R-factor	0.1924
Rwork	0.190
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ih9
RMSD bond length	0.008
RMSD bond angle	1.147
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.202	41.202	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.034	0.654
Rmeas	0.148
Rpim	0.055	0.013	0.256
Total number of observations	121345	5395	16903
Number of reflections	17932
<I/σ(I)>	14.6	41.4	4.3
Completeness [%]	87.7	99.4	81.2
Redundancy	6.8	7.2	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	PEG8000, ethylene glycol