5DJ0

Fc Heterodimer Design 11.2 Y349S/K370Y + E357D/S364Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	193
Detector technology	CCD
Collection date	2013-10-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	60.417, 67.340, 78.308
Unit cell angles	90.00, 104.70, 90.00

Refinement procedure

Resolution	30.000 - 2.280
R-factor	0.19621
Rwork	0.193
R-free	0.25087
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.265
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	75.744	2.410
High resolution limit [Å]	2.280	7.220	2.280
Rmerge		0.029	0.445
Rmeas	0.097
Rpim	0.050	0.020	0.317
Total number of observations	103367	3208	15235
Number of reflections	27745
<I/σ(I)>	11.1	26.5	2.5
Completeness [%]	99.6	98.4	100
Redundancy	3.7	3.5	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	13% PEG 3350 + 200mM Magnesium Formate dihydrate