5DIZ
Crystal Structure of nuclear proteinaceous RNase P 2 (PRORP2) from A. thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.980, 76.993, 80.112 |
| Unit cell angles | 72.66, 64.11, 77.76 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.23042 |
| Rwork | 0.228 |
| R-free | 0.27171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g24 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.654 | |
| Number of reflections | 23244 | |
| <I/σ(I)> | 8.7 | 1.4 |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.8 | 277 | 19.9 % (w/v) PEG3000, and 0.21 M sodium citrate |
