5DIN
Structural Basis for the Indispensable Role of a Unique Zinc Finger Motif in LNX2 Ubiquitination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.969, 69.399, 52.249 |
| Unit cell angles | 90.00, 115.41, 90.00 |
Refinement procedure
| Resolution | 24.240 - 1.864 |
| R-factor | 0.1933 |
| Rwork | 0.190 |
| R-free | 0.23210 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.240 |
| High resolution limit [Å] | 1.864 |
| Number of reflections | 25094 |
| <I/σ(I)> | 9.91 |
| Completeness [%] | 98.9 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M MES monohydrate, 12% w/v Polyethylene glycol 20000 |
