5DIL
Crystal structure of the effector domain of the NS1 protein from influenza virus B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.689, 41.666, 49.681 |
| Unit cell angles | 68.72, 82.83, 78.12 |
Refinement procedure
| Resolution | 28.079 - 2.010 |
| R-factor | 0.1986 |
| Rwork | 0.194 |
| R-free | 0.24250 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.220 |
| High resolution limit [Å] | 2.010 |
| Rmerge | 0.084 |
| Number of reflections | 16041 |
| <I/σ(I)> | 12.22 |
| Completeness [%] | 95.0 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.2M KI 20% PEG3350 |
