5DIF

Crystal Structure of CPEB4 NES Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2014-11-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	105.993, 105.993, 304.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.193 - 2.092
R-factor	0.1716
Rwork	0.170
R-free	0.20840
RMSD bond length	0.003
RMSD bond angle	0.689
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.081	0.022	0.987
Rmeas	0.084	0.024
Rpim	0.035	0.010	0.434
Total number of observations	619523
Number of reflections	102512
<I/σ(I)>	7.9
Completeness [%]	99.5	95.4	100
Redundancy	6	5.7	6.1
CC(1/2)		0.998	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	17% PEG3350, 100mM Bis-Tris pH6.4, 200mM NH4NO3, 10mM Spermine HCl