5DIC
Fatty acid binding protein OBP56a from the oral disk of the blowfly Phormia regina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.845, 33.800, 35.399 |
| Unit cell angles | 105.35, 106.44, 113.12 |
Refinement procedure
| Resolution | 28.100 - 1.185 |
| R-factor | 0.13286 |
| Rwork | 0.131 |
| R-free | 0.16783 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.481 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.100 | 1.220 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.043 | 0.176 |
| Number of reflections | 34518 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 87.4 | |
| Redundancy | 2.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 20% PEG 6000, 1M LiCl, 100mM citrate |
