5DHV
HIV-1 Rev NTD dimers with variable crossing angles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2010-07-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.895, 81.869, 98.809 |
| Unit cell angles | 90.00, 101.09, 90.00 |
Refinement procedure
| Resolution | 20.280 - 2.300 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3X7L |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.846 | |
| Number of reflections | 30611 | |
| <I/σ(I)> | 11.5 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Crystals of scFv-Rev were initially grown in 20% PEG 3350, a variety of salts (200 mM sodium sulfate, sodium bromide, or ammonium phosphate dibasic), and pH ranging from 6.5-8.5. |
