5DHM
Crystal structure of the fimbrial protein Mfa4 from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.973 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.683, 84.536, 138.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.539 - 1.900 |
| R-factor | 0.1859 |
| Rwork | 0.183 |
| R-free | 0.22950 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.124 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.540 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.442 |
| Number of reflections | 327588 | |
| <I/σ(I)> | 16 | 3.4 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 5% polyglutamic acid (PGA), 20% PEG4000 and 0.1 M Tris pH 8.0. Protein was treated with a-chymotrypsin before crystallization. |
