5DHB
Cooperativity and Downstream Binding in RNA Replication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2014-10-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 3 |
| Unit cell lengths | 51.248, 51.248, 38.764 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.380 - 1.800 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.19200 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 3.362 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.380 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.079 | 0.587 |
| Number of reflections | 10344 | |
| <I/σ(I)> | 29.95 | 2.13 |
| Completeness [%] | 98.3 | 94.3 |
| Redundancy | 10.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Magnesium chloride hexahydrate, 30% v/v Polyethylene glycol monomethyl ether 550 |
