5DGO
Crystal structure of cell division cycle protein 45 (Cdc45)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 112.795, 112.795, 143.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.840 - 2.100 |
| R-factor | 0.1693 |
| Rwork | 0.168 |
| R-free | 0.19530 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.620 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.840 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 1.042 | |
| Number of reflections | 61965 | |
| <I/σ(I)> | 11.8 | 2.1 |
| Completeness [%] | 99.8 | 97.1 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | Buffer condition C2 of the Morpheus HT 96 crystallisation screen (Molecular Dimensions) |
