5DGF

Complex of yeast 80S ribosome with hypusine-containing/non-modified eIF5A and/or a peptidyl-tRNA analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.148
Spacegroup name	P 1 21 1
Unit cell lengths	435.580, 287.330, 303.820
Unit cell angles	90.00, 98.95, 90.00

Refinement procedure

Resolution	99.535 - 3.300
R-factor	0.252
Rwork	0.251
R-free	0.30970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v88
RMSD bond length	0.006
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.535		3.400
High resolution limit [Å]	3.300	75.000	3.300
Rmerge	0.590	0.069	2.815
Rmeas	0.625	0.084	3.061
Total number of observations	8339030
Number of reflections	1103604	56	94195
<I/σ(I)>	6	14.18	0.99
Completeness [%]	100.0	50.9	100
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000