5DG4

Crystal structure of monomer human cellular retinol binding protein II-Y60L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	200
Detector technology	CCD
Collection date	2014-11-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	29.526, 36.224, 129.086
Unit cell angles	84.34, 80.26, 67.47

Refinement procedure

Resolution	30.055 - 1.500
R-factor	0.2246
Rwork	0.224
R-free	0.24340
RMSD bond length	0.007
RMSD bond angle	1.073
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	77790
<I/σ(I)>	43.37
Completeness [%]	94.7	94.6
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	30% PEG 4000, 0.1 M sodium acetate, 0.1 M ammonium acetate, pH 4.6