5DFX
Structure of the parental state of GAF3 from Slr1393 of Synechocystis sp. PCC6803 (in vivo assembled protein/chromophore)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.120, 63.120, 118.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.800 |
| R-factor | 0.16585 |
| Rwork | 0.164 |
| R-free | 0.19902 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.428 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.301 |
| Number of reflections | 22947 | |
| <I/σ(I)> | 19.19 | 5.42 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 6.63 | 6.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 285 | 0.02 M Tris pH 7.0, 0.1 M sodium chloride, 7.7 % (w/v) PEG 4000 |
